{"id":17380,"date":"2022-08-28T06:21:13","date_gmt":"2022-08-28T06:21:13","guid":{"rendered":"http:\/\/www.pidsphil.org\/home\/?page_id=17380"},"modified":"2025-06-14T04:28:09","modified_gmt":"2025-06-14T04:28:09","slug":"journal-2021-vol-22-no-2-original-articles-4","status":"publish","type":"page","link":"https:\/\/www.pidsphil.org\/home\/journal-2021-vol-22-no-2-original-articles-4\/","title":{"rendered":"Journal 2021 Vol.22 No.2 Original Articles 4"},"content":{"rendered":"<p><strong><span style=\"color: #800000;\">Journal 2021 Vol.22 No.2<\/span><\/strong><br \/>\n<strong>Rapid Detection of Respiratory Pathogens Using a Multiplex PCR Assay Among Hospitalized Children with Acute Respiratory Infection<\/strong><br \/>\n<strong><em>Katherine B. Javier, M.D., Josephine Anne Navoa-Ng, M.D. and Nikki Cotoco-Chu, M.D.<\/em><br \/>\nAbstract<br \/>\nBackground:<\/strong> Acute respiratory infection (ARI) is a major cause of morbidity and mortality among children worldwide however, local data on the etiologic diagnosis of ARI are limited.<br \/>\n<strong>Objectives:<\/strong> To determine the prevalence and the most commonly detected respiratory pathogens using a multiplex PCR assay, known as the Respiratory Panel, among hospitalized children with ARI and compare their clinical and laboratory differences.<br \/>\n<strong>Methods:<\/strong> This is a cross sectional study of children with ARI who were tested with a multiplex PCR assay. Retrospective chart review was done on these patients admitted from January 2018 to February 2020.<br \/>\n<strong>Results:<\/strong> There were 47 charts reviewed, mean age was 4.2 years old. Out of 47 patients, 36 (76.6%) tested positive for a pathogen. Respiratory syncytial virus (RSV) being the most common followed by Influenza A\/H1-2009 and Human metapneumovirus (hMPV). Two patients had viral co-infections and no bacteria were detected on all subjects. 61.7% patients were started on antibiotics on admission. Fever and cough were the most common sign and symptom, respectively. Normal WBC (68% with neutrophilic predominance) and platelet were detected in 72.3% and 70.2% of patients, respectively; 50% of patients had normal CRP and 60.5% had abnormal findings on chest x-ray. Only the presence of chest x-ray findings was found to have a higher probability of yielding a positive Respiratory Panel p=0.27.<br \/>\n<strong>Conclusion:<\/strong> Among admitted patients with ARI, 76.6% tested positive for a respiratory pathogen. All were caused by viruses presenting as nonspecific manifestations &#8211; fever and cough. Clinical manifestations, CBC and CRP showed no association with the Respiratory Panel result while abnormal chest x-ray had a higher probability of yielding a positive Respiratory Panel result.<br \/>\n<strong>Keywords:<\/strong> Acute respiratory infection, Respiratory Panel, Multiplex PCR assay<br \/>\n<strong><a href=\"https:\/\/doi.org\/10.56964\/pidspj20212202008\">https:\/\/doi.org\/10.56964\/pidspj20212202008<\/a><\/strong><br \/>\n<strong><a href=\"https:\/\/www.pidsphil.org\/home\/wp-content\/uploads\/2022\/12\/008_vol-22-no-2_JAVIER_RESPI-PANEL.pdf\" target=\"_\" rel=\"noopener\">View Full Article in PDF format<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Journal 2021 Vol.22 No.2 Rapid Detection of Respiratory Pathogens Using a Multiplex PCR Assay Among Hospitalized Children with Acute Respiratory Infection Katherine B. Javier, M.D., Josephine Anne Navoa-Ng, M.D. and Nikki Cotoco-Chu, M.D. Abstract Background: Acute respiratory infection (ARI) is a major cause of morbidity and mortality among children worldwide however, local data on the  [&#8230;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-17380","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/pages\/17380","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/comments?post=17380"}],"version-history":[{"count":7,"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/pages\/17380\/revisions"}],"predecessor-version":[{"id":20001,"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/pages\/17380\/revisions\/20001"}],"wp:attachment":[{"href":"https:\/\/www.pidsphil.org\/home\/wp-json\/wp\/v2\/media?parent=17380"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}